山羊催乳素基因PCR-SSCP分析

设计5对引物，采用PCR SSCP技术检测催乳素（prolactin，PRL）基因外显子1~外显子5在高繁殖力山羊品种（济宁青山羊、波尔山羊、文登奶山羊）和低繁殖力山羊品种（内蒙古绒山羊）中的单核苷酸多态性，同时研究该基因对济宁青山羊高繁殖力的影响。通过克隆测序首次获得了山羊PRL基因外显子全序列。结果表明PRL基因在研究的4个山羊品种中都不存在单核苷酸多态性，提示检测的PRL基因座位对济宁青山羊高繁殖力没有显著影响。     

猪囊尾蚴特异性单克隆抗体的制备及其识别抗原成分的鉴定

通过SDS-PAGE方法对猪囊尾蚴囊液抗原成分进行了分离鉴定,并分别分离纯化了其中的16 000和10 000特异性蛋白质成分;将16 000和10 000纯化蛋白质成分分别做成佛氏佐剂苗,分组免疫BALB/c小鼠,超免后,无菌取脾脏制备脾细胞,分别与NS0骨髓瘤细胞进行融合,经阳性筛选和特异性鉴定获得2株分泌猪囊尾蚴特异性单抗的杂交瘤细胞,命名为TSCF-1611H12B8和TSCF-1012G5B5。免疫学鉴定结果表明,单抗TSCF-1611H12B8和TSCF-1012G5B5与猪细颈囊尾蚴、旋毛虫、住肉孢子虫和蛔虫抗原间不存在交叉反应;单抗TSCF-1611H12B8识别囊液中16 000和10 000蛋白质抗原条带,而单抗TSCF-1012G5B5识别囊液中的10 000蛋白质条带。     

番茄细菌性溃疡病菌的实时荧光PCR检测

 由Clavibacter michiganensis subsp.michiganensis(Cmm)引起的番茄细菌性溃疡病是一种严重危害番茄生产的种传细菌性病害。根据ITS序列多态性设计引物及TaqMan探针进行实时荧光PCR检测的结果表明,这组引物一探针能检测出所有供试的Cmm菌,对照菌均未检测到荧光信号。用接种但未显示症状的番茄苗叶片及人工处理的带菌种子提取的核酸作为模板,均能检测到病菌,其检测灵敏度比常规PCR高约100倍。实验中不需病原菌的分离培养及PCR的后续处理。该方法快速、简便、安全、准确,适用于出入境检验检疫及种子、种苗健康检测领域。     

表达猪繁殖与呼吸综合征病毒CH-1a株核衣壳蛋白重组鸡痘病毒的构建

从含有猪繁殖与呼吸综合征病毒（PRRSV）核衣壳蛋白（N）的质粒扩增出N基因，构建禽痘病毒转移载体。该载体含有禽痘病毒早晚期启动子LP2EP2控制之下的PRRSVN基因、P11启动下的报告基因lacZ以及用于同源重组的禽痘病毒基因组的片段。在转移载体转染亲本病毒S—FPV-017感染的鸡胚成纤维细胞（CEF）之后，采用蓝色表型筛选的方法，筛选到表达N基因的重组病毒，并对其进行了6轮蚀斑纯化。PCR方法鉴定证明重组病毒的基因组中含有完整PRRSVN基因，间接免疫荧光试验证明了PRRSVN蛋白在重组病毒感染的CEF细胞中获得表达，本研究为猪繁殖与呼吸综合征非复制型疫苗的研制打下了基础。     

The relationship between serum IGF-1, IGFBP-3 levels,bone mineral density and bone metabolic markers in postmenopausal patients with osteoporosis

AIM:To study the relationship between serum insulin-like growth factor- 1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3) levels, bone mineral density(BMD) and bone metabolic markers in postmenopausal women. METHODS:Serum IGF-1, IGFBP-3, osteocalcin(BGP), isomeric C-telopeptide of type I collagen (β-CTX), estradiol(E₂), calcitonin(CT), parathormone(PTH), calcium (Ca), and phosphorus(P) were measured in 90 postmenopausal osteoporosis patients and 70 healthy postmenopausal women. BMD of lumbar vertebra and left femoral neck were determined by dual energy X-ray absorptiometry.RESULTS:BMD of lumbar vertebra and left femoral neck decreased significantly (P<0.01), serum IGF-1, IGFBP-3, E₂, CT and BGP decreased significantly (P<0.01), serum β-CTX and PTH increased significantly in postmenopausal osteoporosis group (P<0.01). There were no significantly differences in serum Ca, P between two groups (P>0.05). BMD of lumbar vertebra and left femoral neck were positively correlated with serum IGF-1, IGFBP-3, E₂, CT and BGP, but negatively correlated with β CTX and PTH. There were no correlation with serum Ca, P and BMD.CONCLUSION:Serum IGF-1, IGFBP-3, E₂, CT, BGP, β-CTX and PTH level were correlated with BMD of lumbar vertebra and left femoral neck. These markers can be one of the valuable evidences for screening osteoporosis in postmenopausal women.     

土壤硫铁元素偏高对玉米自交系生殖生长影响研究

通过调查取样,化验分析,在土壤硫铁元素比对照超过144.00%和54.19%的条件下,对玉米自交系(苏37-2)造成不吐丝现象进行了调查研究.     

基于进化半监督模糊聚类算法的病毒检测研究

现有的计算机病毒检测技术很难检测出未知病毒,在病毒防御中处于被动.复杂的病毒形式,迫切需要一种具有自学习能力,能主动分类、识别和检测未知病毒的方法.分析现有的病毒检测技术,研究进化半监督模糊聚类算法在病毒检测中的应用,探讨其关键技术,在此基础上给出一种病毒检测模型,并通过计算机仿真进行验证,实验结果表明新的方法对未知病毒检测是有效的.     

蔬菜保护地秸秆施用技术与效果

针对绥中县蔬菜保护地因连年生产,大量施用化肥而造成的土壤肥力下降等一系列问题,以及当地玉米秸秆利用率低的情况,引进了保护地秸秆还田技术.介绍秸秆还田技术的操作要点和主要作用,分析该技术推广应用后取得的显著经济效益及社会效益.     

豫西山区野生百合中层鳞片扦插繁殖技术研究

用混合基质、砂、田土3种基质对豫西山区5种野生百合中层鳞片进行扦插繁殖试验.结果表明:5种野生百合中层鳞片都具有较强的扦插繁殖能力,同时证明5种野生百合中层鳞片在不同基质中的扦插成活与成球情况不同,混合基质最适宜野生百合中层鳞片扦插繁殖.     

Impact of weedy rice populations on the growth and yield of direct-seeded and transplanted rice

To examine the impact of weedy rice ( Oryza sativa L. f. spontanea ) populations on the growth and yield of direct-seeded and transplanted rice ( Oryza sativa ), a field experiment with a random two-factor design that included cultivation methods (direct-seeding and transplanting) and the density of weedy rice (0, 5, 25, and 125 plants per m²) was conducted. The data from the experiment showed that weedy rice had a significantly poorer performance in the direct-seeded fields than in the transplanted fields in terms of its vegetative (plant height) and reproductive traits (panicle and seed production). In contrast, with the interference of weedy rice, cultivated rice showed an improved performance in the direct-seeded fields than in the transplanted fields, with a significantly higher tiller production and grain yield. The results suggest that cultivated rice can tolerate more successfully the infestation of weedy rice in direct-seeded fields because of its enhanced competitive ability compared to that in transplanted fields.